He attended Wesleyan University, where he had initially planned to pursue a career in Medicine followed by his identical twin brother Andy, who later became a urologist. After taking a course on film history, Sher developed an immediate interest in cinema, recalling that "All I wanted to do was spend every waking moment learning about movies and cinematography." He decided on majoring in economics and graduated from Wesleyan in 1992.
After graduating from college, he moved to Los Angeles and immediately pursued a career in motion pictures. He started as a gofer on a video shoot and an assistant on commercials before working his way up to Cinematographer. Sher's first major release was the 2001 film Kissing Jessica Stein. He achieved critical success with the 2004 movie Garden State. Duane Byrge of The Hollywood Reporter, in his review for the 2004 Sundance Film Festival, credited Sher's compositions with "pack[ing] insight into the character's psychological states". Sher also has worked on 2007's Dan in Real Life starring Steve Carell and Juliette Binoche and the 2009 release I Love You, Man with Paul Rudd. While Sher aspires to direct, he is in no rush given that "I don't think I’ve fulfilled all of my goals as a Cinematographer yet".
As Cinematographer of the 2009 film The Hangover, Sher described how a scene early in the movie shows the main characters on the roof of their hotel overlooking a stereotypical shot of the Las Vegas Strip; Sher indicated that he had tried to evoke the behind-the-scenes Vegas—after the characters wake up the following morning—by shooting a scene behind the hotels where the real action takes place. Actor Bradley Cooper credited Sher's visual style with enhancing the film's comedy, noting how Sher has "a great eye, a lot of Energy and he just knows what's funny" and that "Some guys just can’t shoot comedies, but Larry knows exactly what he's doing."
With the success of Zach Braff's Kickstarter, Sher worked on Braff's 2014 feature, Wish I Was Here.
As of 2017, Sher lives in Los Angeles, California, together with his partner Hema Patel and his 12 year-old son, Max, and daughter Matilda. He was previously married to Jessica Aronoff from 1998 to 2017.
