Her first audition won her the role of Kirilenko in David Cronenberg's Eastern Promises (2007) starring Viggo Mortensen and Vincent Cassel. Soon after she has appeared in films St. Trinian's (alongside Russell Brand), Inkheart (starring Brendan Fraser and Paul Bettany) and Eichmann (starring Thomas Kretschmann).
In 2010, Srbová played the lead role of Silka in the Lionsgate horror Siren, with Eoin Macken and Anna Skelern. In this film, Srbová performed the song Elephants by Warpaint (band).
In 2013, Srbová played a re-occurring role of an agent Major Nina Pirogova in Cinemax/HBO series Strike Back: Shadow Warfare starring Philip Winchester and Sullivan Stapleton. In 2015 she reprised her role in Strike Back: Legacy.
