Glazer began taking classes at the Upright Citizens Brigade Theatre in 2006 and performed around New York City doing improv and stand-up for the next several years.
She graduated from New York University in 2009, majoring in psychology. She was once roommates with Rachel Bloom after college in Brooklyn. She revealed to The New Yorker that she smokes marijuana every day, but rarely drinks alcohol. In February 2017, Glazer married her longtime boyfriend, computational Biologist David Rooklin, in a private ceremony.
In 2011, cable network FX, working with Poehler as the Producer, purchased a script commitment for the series from Glazer and Jacobson. However, the network did not approve the script and decided not to proceed with development. Glazer and Jacobson then approached Comedy Central, who agreed to purchase the script from FX and order a pilot.
Glazer starred in the 2013 independent feature film, How to Follow Strangers. The film won top prize at the Lower East Side Film Festival. She appears in the 2015 film The Night Before. Glazer starred alongside Scarlett Johansson and Kate McKinnon in the 2017 comedy Rough Night.
In February 2014, Comedy Central renewed the show for a second season. Season 2 received positive reviews, with Metacritic giving it a score of 89 out of 100, based on reviews from 8 critics, indicating "universal acclaim."
In January 2015, the series was then renewed for a third season, which premiered on February 17, 2016. In January 2016, the series was renewed for a fourth and a fifth season.
