In 2005, she represented Antrim in the Rose of Tralee competition. Wisener also was part of Musical Theatre 4 Youth, and appeared in a workshop production of Falling.
Wisener made her stage debut in the UK premiere of the musical Parade at the Donmar Warehouse theatre in 2007.
She appeared in Boogeyman 3 playing the role of Amy and in November 2008 created the role of Sophie in a West End showcase of the new musical The Lost Christmas by Laurence Mark Wythe at the Trafalgar Studios, London.
In late 2009, until early 2010 she appeared on stage playing the role of Mary Lennox in The Secret Garden at the West Yorkshire Playhouse.
In March 2011 she played Sally, a young woman in a centre for female Juvenile delinquents, in The Runaway, based on Martina Cole's novel.
From December 2012 to January 2013, Jayne starred as Cinderella at The Grand Opera House, in Belfast in the pantomime, Cinderella.
