Stroker starred in the Paper Mill Playhouse's production of The 25th Annual Putnam County Spelling Bee. This performance earned Ali a Barrymore Award nomination, and remarkable praise when reprised at the Paper Mill Playhouse.
In 2009, Ali became the first Actress in a wheelchair to earn a degree from the New York University Tisch Drama Department, with a degree in Fine Arts.
In 2011, Stroker made an appearance in the short, I Was a Mermaid and Now I'm a Pop Star. Then in 2012, Stroker auditioned for The Glee Project and was cast for the 12 episode series. Ali made it to the final episode and placed second, earning a guest role on Glee. In 2013, Ali played Betty Pillsbury, Ms. Pilsbury's niece on Glee in Season 4, Episode 14 "I Do".
In 2014, Stroker gained a role in the film Cotton alongside Gary Cole. Later on in 2014 and into 2015, Stroker earned a three episode role playing the character Wendy in MTV's series, Faking It.
In 2015 Stroker made history by becoming the first Broadway Actress in a wheelchair to appear on a Broadway stage. She originated the role of Anna in Deaf West's 2015 revival of Spring Awakening.
In 2017, Stroker was cast as Tamara in the upcoming ABC show Ten Days in the Valley.