She has performed off-Broadway, in regional Shakespeare, and on several TV shows. She began in national television in 2000 in the role of Rainn Wilkins on several seasons of All My Children. Then in 2002 she returned to theater on Broadway where she debuted in the original stage cast of The Graduate as an understudy for several roles, eventually replacing Alicia Silverstone in the role of Elaine Robinson, opposite Kathleen Turner and Jason Biggs.
Overton married fellow actor Judson Pearce Morgan in March 2004. In 2011 Overton gave birth to their daughter. It was confirmed during San Diego Comic-Con 2017 that Overton was pregnant during the production of the second season of Van Helsing. Overton gave birth to her second daughter in July 2017.
After five years in New York she moved to Los Angeles, where she has worked in film and TV. In 2008, with husband Judson Pearce Morgan, she wrote, directed, produced and starred in The Collective. She plays Christie Monteiro in the 2009 film adaptation of the fighting game series Tekken. She recently appeared in the HBO series True Blood as werewolf Rikki Naylor.
In 2016 Overton was cast as Vanessa Helsing, the lead character, in the Syfy television series, Van Helsing.
